The effect of blood-flow-restricted interval training on lactate and H+ dynamics during dynamic exercise in man
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The effect of blood-flow-restricted interval training on lactate and H+ dynamics during dynamic exercise in man. / Christiansen, Danny; Eibye, Kasper; Hostrup, Morten; Bangsbo, Jens.
I: Acta Physiologica (Print), Bind 231, Nr. 3, e13580, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The effect of blood-flow-restricted interval training on lactate and H+ dynamics during dynamic exercise in man
AU - Christiansen, Danny
AU - Eibye, Kasper
AU - Hostrup, Morten
AU - Bangsbo, Jens
N1 - This article is protected by copyright. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Aim: To assess how blood-flow-restricted (BFR) interval-training affects the capacity of the leg muscles for pH regulation during dynamic exercise in physically-trained men.Methods: Ten men (age: 25 ±4y; V̇O2max: 50 ±5mL∙kg-1∙min-1) completed a 6-wk interval-cycling intervention (INT) with one leg under BFR (BFR-leg; ~180 mmHg) and the other without BFR (CON-leg). Before and after INT, thigh net H+-release (lactate-dependent, lactate-independent, and sum) and blood acid/base variables, were measured during knee-extensor-exercise at 25% (Ex25) and 90% (Ex90) of incremental-peak-power-output. A muscle biopsy was collected before and after Ex90 to determine pH, lactate, and density of H+ -transport/buffering systems.Results: After INT, net H+-release (BFR-leg: 15±2; CON-leg: 13±3; mmol·min-1; Mean±95%CI), net lactate-independent H+ -release (BFR-leg: 8±1; CON-leg: 4±1; mmol·min-1), and net lactate-dependent H+-release (BFR-leg: 9±3; CON-leg: 10±3; mmol·min-1) were similar between legs during Ex90 (p>0.05), despite a ~142% lower muscle-intracellular-to-interstitial lactate-gradient in BFR-leg (-3±4 vs. 6±6 mmol·L-1 ; p<0.05). In recovery from Ex90, net lactate-dependent H+-efflux decreased in BFR-leg with INT (p<0.05 vs. CON-leg) due to lowered muscle lactate production (~58% vs. CON-leg, p<0.05). Net H+ -gradient was not different between legs (~19%, p>0.05; BFR-leg: 48±30; CON-leg: 44±23; mmol·L-1). In BFR-leg, NHE1-density was higher than in CON-leg (~45%; p<0.05) and correlated with total-net H+-release (r=0.71; p=0.031) and lactate-independent H+-release (r=0.74; p=0.023) after INT, where arterial [HCO3-] and standard-base-excess in Ex25 were higher in BFR-leg than CON-leg.Conclusion: Compared to a training control, BFR-interval training increases the capacity for pH-regulation during dynamic exercise mainly via enhancement of muscle lactate-dependent H+-transport function and blood H+-buffering capacity.
AB - Aim: To assess how blood-flow-restricted (BFR) interval-training affects the capacity of the leg muscles for pH regulation during dynamic exercise in physically-trained men.Methods: Ten men (age: 25 ±4y; V̇O2max: 50 ±5mL∙kg-1∙min-1) completed a 6-wk interval-cycling intervention (INT) with one leg under BFR (BFR-leg; ~180 mmHg) and the other without BFR (CON-leg). Before and after INT, thigh net H+-release (lactate-dependent, lactate-independent, and sum) and blood acid/base variables, were measured during knee-extensor-exercise at 25% (Ex25) and 90% (Ex90) of incremental-peak-power-output. A muscle biopsy was collected before and after Ex90 to determine pH, lactate, and density of H+ -transport/buffering systems.Results: After INT, net H+-release (BFR-leg: 15±2; CON-leg: 13±3; mmol·min-1; Mean±95%CI), net lactate-independent H+ -release (BFR-leg: 8±1; CON-leg: 4±1; mmol·min-1), and net lactate-dependent H+-release (BFR-leg: 9±3; CON-leg: 10±3; mmol·min-1) were similar between legs during Ex90 (p>0.05), despite a ~142% lower muscle-intracellular-to-interstitial lactate-gradient in BFR-leg (-3±4 vs. 6±6 mmol·L-1 ; p<0.05). In recovery from Ex90, net lactate-dependent H+-efflux decreased in BFR-leg with INT (p<0.05 vs. CON-leg) due to lowered muscle lactate production (~58% vs. CON-leg, p<0.05). Net H+ -gradient was not different between legs (~19%, p>0.05; BFR-leg: 48±30; CON-leg: 44±23; mmol·L-1). In BFR-leg, NHE1-density was higher than in CON-leg (~45%; p<0.05) and correlated with total-net H+-release (r=0.71; p=0.031) and lactate-independent H+-release (r=0.74; p=0.023) after INT, where arterial [HCO3-] and standard-base-excess in Ex25 were higher in BFR-leg than CON-leg.Conclusion: Compared to a training control, BFR-interval training increases the capacity for pH-regulation during dynamic exercise mainly via enhancement of muscle lactate-dependent H+-transport function and blood H+-buffering capacity.
KW - Faculty of Science
KW - Blood flow restriction
KW - Training
KW - H+ release
KW - pH regulation
KW - Lactate
KW - Performance
KW - Cycling
KW - Exercise
U2 - 10.1111/apha.13580
DO - 10.1111/apha.13580
M3 - Journal article
C2 - 33222371
VL - 231
JO - Acta Physiologica
JF - Acta Physiologica
SN - 1748-1708
IS - 3
M1 - e13580
ER -
ID: 251936655