Sequence specific inhibition of DNA restriction enzyme cleavage by PNA
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Sequence specific inhibition of DNA restriction enzyme cleavage by PNA. / Nielsen, Peter E.; Egholm, M; Berg, Rolf H.; Buchardt, O.
I: Nucleic Acids Research, Bind 21, Nr. 2, 25.01.1993, s. 197-200.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Sequence specific inhibition of DNA restriction enzyme cleavage by PNA
AU - Nielsen, Peter E.
AU - Egholm, M
AU - Berg, Rolf H.
AU - Buchardt, O
PY - 1993/1/25
Y1 - 1993/1/25
N2 - Plasmids containing double-stranded 10-mer PNA (peptide nucleic acid chimera) targets proximally flanked by two restriction enzyme sites were challenged with the complementary PNA or PNAs having one or two mismatches, and the effect on the restriction enzyme cleavage of the flanking sites was assayed. The following PNAs were used: T10-LysNH2, T5CT4-LysNH2 and T2CT2CT4-LysNH2 and the corresponding targets cloned into pUC 19 were flanked by BamH1, Sal1 or Pstl sites, respectively. In all cases it was found that complete inhibition of restriction enzyme cleavage was obtained with the complementary PNA, a significantly reduced effect was seen with a PNA having one mismatch, and no effect was seen with a PNA having two mismatches. These results show that PNA can be used as sequence specific blockers of DNA recognizing proteins.
AB - Plasmids containing double-stranded 10-mer PNA (peptide nucleic acid chimera) targets proximally flanked by two restriction enzyme sites were challenged with the complementary PNA or PNAs having one or two mismatches, and the effect on the restriction enzyme cleavage of the flanking sites was assayed. The following PNAs were used: T10-LysNH2, T5CT4-LysNH2 and T2CT2CT4-LysNH2 and the corresponding targets cloned into pUC 19 were flanked by BamH1, Sal1 or Pstl sites, respectively. In all cases it was found that complete inhibition of restriction enzyme cleavage was obtained with the complementary PNA, a significantly reduced effect was seen with a PNA having one mismatch, and no effect was seen with a PNA having two mismatches. These results show that PNA can be used as sequence specific blockers of DNA recognizing proteins.
KW - Base Sequence
KW - Chimera
KW - DNA
KW - DNA Restriction Enzymes/antagonists & inhibitors
KW - Deoxyribonuclease BamHI/antagonists & inhibitors
KW - Deoxyribonucleases, Type II Site-Specific/antagonists & inhibitors
KW - Molecular Sequence Data
KW - Oligodeoxyribonucleotides/metabolism
KW - Peptides/metabolism
KW - Restriction Mapping
U2 - 10.1093/nar/21.2.197
DO - 10.1093/nar/21.2.197
M3 - Journal article
C2 - 8382793
VL - 21
SP - 197
EP - 200
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 2
ER -
ID: 203630933