PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules

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Standard

PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. / Buchardt, O; Freier, S M; Driver, D A; Kim, S K; Norden, B; Nielsen, Peter E.

I: Nature, Bind 365, Nr. 6446, 07.10.1993, s. 566-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Buchardt, O, Freier, SM, Driver, DA, Kim, SK, Norden, B & Nielsen, PE 1993, 'PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules', Nature, bind 365, nr. 6446, s. 566-8. https://doi.org/10.1038/365566a0

APA

Buchardt, O., Freier, S. M., Driver, D. A., Kim, S. K., Norden, B., & Nielsen, P. E. (1993). PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. Nature, 365(6446), 566-8. https://doi.org/10.1038/365566a0

Vancouver

Buchardt O, Freier SM, Driver DA, Kim SK, Norden B, Nielsen PE. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. Nature. 1993 okt. 7;365(6446):566-8. https://doi.org/10.1038/365566a0

Author

Buchardt, O ; Freier, S M ; Driver, D A ; Kim, S K ; Norden, B ; Nielsen, Peter E. / PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules. I: Nature. 1993 ; Bind 365, Nr. 6446. s. 566-8.

Bibtex

@article{766ed0322bda4df795176db73b038cac,
title = "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules",
abstract = "DNA analogues are currently being intensely investigated owing to their potential as gene-targeted drugs. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes, and bind to double-stranded DNA by strand displacement. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.",
keywords = "Base Sequence, Cytosine/chemistry, DNA/chemistry, Glycine/analogs & derivatives, Hydrogen Bonding, Molecular Sequence Data, Nucleic Acid Hybridization, Oligodeoxyribonucleotides/chemistry, RNA/chemistry, Thermodynamics, Thymine/chemistry",
author = "O Buchardt and Freier, {S M} and Driver, {D A} and Kim, {S K} and B Norden and Nielsen, {Peter E.}",
year = "1993",
month = oct,
day = "7",
doi = "10.1038/365566a0",
language = "English",
volume = "365",
pages = "566--8",
journal = "Nature",
issn = "0028-0836",
publisher = "nature publishing group",
number = "6446",

}

RIS

TY - JOUR

T1 - PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules

AU - Buchardt, O

AU - Freier, S M

AU - Driver, D A

AU - Kim, S K

AU - Norden, B

AU - Nielsen, Peter E.

PY - 1993/10/7

Y1 - 1993/10/7

N2 - DNA analogues are currently being intensely investigated owing to their potential as gene-targeted drugs. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes, and bind to double-stranded DNA by strand displacement. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.

AB - DNA analogues are currently being intensely investigated owing to their potential as gene-targeted drugs. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes, and bind to double-stranded DNA by strand displacement. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.

KW - Base Sequence

KW - Cytosine/chemistry

KW - DNA/chemistry

KW - Glycine/analogs & derivatives

KW - Hydrogen Bonding

KW - Molecular Sequence Data

KW - Nucleic Acid Hybridization

KW - Oligodeoxyribonucleotides/chemistry

KW - RNA/chemistry

KW - Thermodynamics

KW - Thymine/chemistry

U2 - 10.1038/365566a0

DO - 10.1038/365566a0

M3 - Journal article

C2 - 7692304

VL - 365

SP - 566

EP - 568

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6446

ER -

ID: 203630618