Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding

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Standard

Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding. / Lucking, Karl; Nielsen, Jesper M.; Pedersen, Per Amstrup; Jørgensen, Peter L.

I: American Journal of Physiology, Bind 271, Nr. 2 PART 2, 01.12.1996.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lucking, K, Nielsen, JM, Pedersen, PA & Jørgensen, PL 1996, 'Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding', American Journal of Physiology, bind 271, nr. 2 PART 2.

APA

Lucking, K., Nielsen, J. M., Pedersen, P. A., & Jørgensen, P. L. (1996). Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding. American Journal of Physiology, 271(2 PART 2).

Vancouver

Lucking K, Nielsen JM, Pedersen PA, Jørgensen PL. Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding. American Journal of Physiology. 1996 dec. 1;271(2 PART 2).

Author

Lucking, Karl ; Nielsen, Jesper M. ; Pedersen, Per Amstrup ; Jørgensen, Peter L. / Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding. I: American Journal of Physiology. 1996 ; Bind 271, Nr. 2 PART 2.

Bibtex

@article{4fd98900051245f68ce777cae39268db,
title = "Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding",
abstract = "For understand-ing the regulation of sodium reabsorption, it is important to know whether the α2- or α3-isoform of Na-K-adenosinetriphosphatase (Na-K-ATPase) is expressed in mammalian kidney in addition to the predominant a-isozyme. Here we applied competitive polymerase chain reaction (PCR) for estimation of mRNAin parenchymal zones of rat kidney for comparison to high-affinity [3H]ouabain binding. The α3-isoform mRNA was demonstrated to form 0.04-0.05% of the amount of arisoform mRNA in the cortex, medulla, and papilla of rat kidney. The α2-mRNA was demonstrated in all three zones and constituted 0.03% of the amount of arinRNA in cortex. The abundance of the a. truncated mRNA was 0.1-0.8% of that of the ai-mRNA. The upper limit for expression of Na-K-ATPase isozyme with high ouabain affinity (dissociation constant, 69-141 nM) was 0.096-0.14% of the concentration of α1β1-Na-K-ATPase. Thus a small but well-defined pool of α2- and α3-isoforms constitutes £0.1% of the amount of oti-isoform at both the mRNA and protein level in rat kidney.",
keywords = "Ai truncated form; messenger ribonucleic acid levels, Parenchymal zones, Reverse transcription-polymerase chain reaction, Sodium-potassium pump, Sodium-potassium-adenosinetriphosphatase",
author = "Karl Lucking and Nielsen, {Jesper M.} and Pedersen, {Per Amstrup} and J{\o}rgensen, {Peter L.}",
year = "1996",
month = dec,
day = "1",
language = "English",
volume = "271",
journal = "American Journal of Physiology - Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "2 PART 2",

}

RIS

TY - JOUR

T1 - Na-K-ATPase isoform (α3, α2, α1) abundance in rat kidney estimated by competitive RT-PCR and ouabain binding

AU - Lucking, Karl

AU - Nielsen, Jesper M.

AU - Pedersen, Per Amstrup

AU - Jørgensen, Peter L.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - For understand-ing the regulation of sodium reabsorption, it is important to know whether the α2- or α3-isoform of Na-K-adenosinetriphosphatase (Na-K-ATPase) is expressed in mammalian kidney in addition to the predominant a-isozyme. Here we applied competitive polymerase chain reaction (PCR) for estimation of mRNAin parenchymal zones of rat kidney for comparison to high-affinity [3H]ouabain binding. The α3-isoform mRNA was demonstrated to form 0.04-0.05% of the amount of arisoform mRNA in the cortex, medulla, and papilla of rat kidney. The α2-mRNA was demonstrated in all three zones and constituted 0.03% of the amount of arinRNA in cortex. The abundance of the a. truncated mRNA was 0.1-0.8% of that of the ai-mRNA. The upper limit for expression of Na-K-ATPase isozyme with high ouabain affinity (dissociation constant, 69-141 nM) was 0.096-0.14% of the concentration of α1β1-Na-K-ATPase. Thus a small but well-defined pool of α2- and α3-isoforms constitutes £0.1% of the amount of oti-isoform at both the mRNA and protein level in rat kidney.

AB - For understand-ing the regulation of sodium reabsorption, it is important to know whether the α2- or α3-isoform of Na-K-adenosinetriphosphatase (Na-K-ATPase) is expressed in mammalian kidney in addition to the predominant a-isozyme. Here we applied competitive polymerase chain reaction (PCR) for estimation of mRNAin parenchymal zones of rat kidney for comparison to high-affinity [3H]ouabain binding. The α3-isoform mRNA was demonstrated to form 0.04-0.05% of the amount of arisoform mRNA in the cortex, medulla, and papilla of rat kidney. The α2-mRNA was demonstrated in all three zones and constituted 0.03% of the amount of arinRNA in cortex. The abundance of the a. truncated mRNA was 0.1-0.8% of that of the ai-mRNA. The upper limit for expression of Na-K-ATPase isozyme with high ouabain affinity (dissociation constant, 69-141 nM) was 0.096-0.14% of the concentration of α1β1-Na-K-ATPase. Thus a small but well-defined pool of α2- and α3-isoforms constitutes £0.1% of the amount of oti-isoform at both the mRNA and protein level in rat kidney.

KW - Ai truncated form; messenger ribonucleic acid levels

KW - Parenchymal zones

KW - Reverse transcription-polymerase chain reaction

KW - Sodium-potassium pump

KW - Sodium-potassium-adenosinetriphosphatase

UR - http://www.scopus.com/inward/record.url?scp=0029781307&partnerID=8YFLogxK

M3 - Journal article

C2 - 8770155

AN - SCOPUS:0029781307

VL - 271

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 2 PART 2

ER -

ID: 227044094