A role for giantin in docking COPI vesicles to Golgi membranes

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Standard

A role for giantin in docking COPI vesicles to Golgi membranes. / Sönnichsen, B; Lowe, M; Levine, T; Jämsä, E; Dirac-Svejstrup, B; Warren, G.

I: The Journal of Cell Biology, Bind 140, Nr. 5, 09.03.1998, s. 1013-21.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sönnichsen, B, Lowe, M, Levine, T, Jämsä, E, Dirac-Svejstrup, B & Warren, G 1998, 'A role for giantin in docking COPI vesicles to Golgi membranes', The Journal of Cell Biology, bind 140, nr. 5, s. 1013-21. https://doi.org/10.1083/jcb.140.5.1013

APA

Sönnichsen, B., Lowe, M., Levine, T., Jämsä, E., Dirac-Svejstrup, B., & Warren, G. (1998). A role for giantin in docking COPI vesicles to Golgi membranes. The Journal of Cell Biology, 140(5), 1013-21. https://doi.org/10.1083/jcb.140.5.1013

Vancouver

Sönnichsen B, Lowe M, Levine T, Jämsä E, Dirac-Svejstrup B, Warren G. A role for giantin in docking COPI vesicles to Golgi membranes. The Journal of Cell Biology. 1998 mar. 9;140(5):1013-21. https://doi.org/10.1083/jcb.140.5.1013

Author

Sönnichsen, B ; Lowe, M ; Levine, T ; Jämsä, E ; Dirac-Svejstrup, B ; Warren, G. / A role for giantin in docking COPI vesicles to Golgi membranes. I: The Journal of Cell Biology. 1998 ; Bind 140, Nr. 5. s. 1013-21.

Bibtex

@article{4d5dd5bdd88042acbc46f200c32763a9,
title = "A role for giantin in docking COPI vesicles to Golgi membranes",
abstract = "We have previously shown that p115, a vesicle docking protein, binds to two proteins (p130 and p400) in detergent extracts of Golgi membranes. p130 was identified as GM130, a Golgi matrix protein, and was shown to act as a membrane receptor for p115. p400 has now been identified as giantin, a Golgi membrane protein with most of its mass projecting into the cytoplasm. Giantin is found on COPI vesicles and pretreatment with antibodies inhibits both the binding of p115 and the docking of these vesicles with Golgi membranes. In contrast, GM130 is depleted from COPI vesicles and inhibition of the GM130 on Golgi membranes, using either antibodies or an NH2-terminal GM130 peptide, inhibits p115 binding and vesicle docking. Together these results suggest that COPI vesicles are docked by giantin on the COPI vesicles and GM130 on Golgi membranes with p115 providing a bridge.",
keywords = "Animals, Autoantigens, Carrier Proteins/metabolism, Coated Vesicles/metabolism, Coatomer Protein, Golgi Apparatus/metabolism, Golgi Matrix Proteins, Intracellular Membranes/metabolism, Membrane Proteins/metabolism, Rats, Vesicular Transport Proteins",
author = "B S{\"o}nnichsen and M Lowe and T Levine and E J{\"a}ms{\"a} and B Dirac-Svejstrup and G Warren",
year = "1998",
month = mar,
day = "9",
doi = "10.1083/jcb.140.5.1013",
language = "English",
volume = "140",
pages = "1013--21",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

RIS

TY - JOUR

T1 - A role for giantin in docking COPI vesicles to Golgi membranes

AU - Sönnichsen, B

AU - Lowe, M

AU - Levine, T

AU - Jämsä, E

AU - Dirac-Svejstrup, B

AU - Warren, G

PY - 1998/3/9

Y1 - 1998/3/9

N2 - We have previously shown that p115, a vesicle docking protein, binds to two proteins (p130 and p400) in detergent extracts of Golgi membranes. p130 was identified as GM130, a Golgi matrix protein, and was shown to act as a membrane receptor for p115. p400 has now been identified as giantin, a Golgi membrane protein with most of its mass projecting into the cytoplasm. Giantin is found on COPI vesicles and pretreatment with antibodies inhibits both the binding of p115 and the docking of these vesicles with Golgi membranes. In contrast, GM130 is depleted from COPI vesicles and inhibition of the GM130 on Golgi membranes, using either antibodies or an NH2-terminal GM130 peptide, inhibits p115 binding and vesicle docking. Together these results suggest that COPI vesicles are docked by giantin on the COPI vesicles and GM130 on Golgi membranes with p115 providing a bridge.

AB - We have previously shown that p115, a vesicle docking protein, binds to two proteins (p130 and p400) in detergent extracts of Golgi membranes. p130 was identified as GM130, a Golgi matrix protein, and was shown to act as a membrane receptor for p115. p400 has now been identified as giantin, a Golgi membrane protein with most of its mass projecting into the cytoplasm. Giantin is found on COPI vesicles and pretreatment with antibodies inhibits both the binding of p115 and the docking of these vesicles with Golgi membranes. In contrast, GM130 is depleted from COPI vesicles and inhibition of the GM130 on Golgi membranes, using either antibodies or an NH2-terminal GM130 peptide, inhibits p115 binding and vesicle docking. Together these results suggest that COPI vesicles are docked by giantin on the COPI vesicles and GM130 on Golgi membranes with p115 providing a bridge.

KW - Animals

KW - Autoantigens

KW - Carrier Proteins/metabolism

KW - Coated Vesicles/metabolism

KW - Coatomer Protein

KW - Golgi Apparatus/metabolism

KW - Golgi Matrix Proteins

KW - Intracellular Membranes/metabolism

KW - Membrane Proteins/metabolism

KW - Rats

KW - Vesicular Transport Proteins

U2 - 10.1083/jcb.140.5.1013

DO - 10.1083/jcb.140.5.1013

M3 - Journal article

C2 - 9490716

VL - 140

SP - 1013

EP - 1021

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -

ID: 333770435