MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
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MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides. / Lindahl, Lise M; Gluud, Maria; Emmanuel, Thomas; Thomsen, Emil A; Hu, Tengpeng; Rittig, Anne H; Celis, Pamela; Stolearenco, Veronica; Krejsgaard, Thorbjørn; Johansen, Claus; Willerslev-Olsen, Andreas; Buus, Terkild B; Woetmann, Anders; Aagaard, Lars; Geisler, Carsten; Litman, Thomas; Mikkelsen, Jacob G; Odum, Niels; Iversen, Lars.
I: Acta Dermato-Venereologica, Bind 100, adv00270, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
AU - Lindahl, Lise M
AU - Gluud, Maria
AU - Emmanuel, Thomas
AU - Thomsen, Emil A
AU - Hu, Tengpeng
AU - Rittig, Anne H
AU - Celis, Pamela
AU - Stolearenco, Veronica
AU - Krejsgaard, Thorbjørn
AU - Johansen, Claus
AU - Willerslev-Olsen, Andreas
AU - Buus, Terkild B
AU - Woetmann, Anders
AU - Aagaard, Lars
AU - Geisler, Carsten
AU - Litman, Thomas
AU - Mikkelsen, Jacob G
AU - Odum, Niels
AU - Iversen, Lars
PY - 2020
Y1 - 2020
N2 - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.
AB - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.
U2 - 10.2340/00015555-3574
DO - 10.2340/00015555-3574
M3 - Journal article
C2 - 32556351
VL - 100
JO - Acta Dermato-Venereologica
JF - Acta Dermato-Venereologica
SN - 0001-5555
M1 - adv00270
ER -
ID: 247511138